Biblioteca Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche
Sanità Pubblica Veterinaria: Numero 112, Febbraio 2019 [http://www.spvet.it/] ISSN 1592-1581
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Smart Poster: SERUM MICRORNAs IN BUFFALO COWS: POTENTIAL BIOMARKERS OF PREGNANCY - Micro RNA serici in femmine di bufalo: potenziali biomarker di gravidanza. [Ver. 1.0]
Tesista: Michela Capurro; Relatore: Danilo Zampini; Correlatore: Gabriella Guelfi, aoul Ciappelloni





Total RNA isolation, reverse transcription, and qPCR amplification

Total RNA, including miRNAs, was extracted from 500 µL of serum using the Plasma Serum Circulating and Exosomal RNA purification mini Kit (Norgen Biotek Corp. Ontario, Canada), slightly modifying manufacturer's recommendations.


Updated bibliography

Livak, K.J., Schmittgen, T.D., 2001. Analysis of relative gene expression data using realtime quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25, 402–408. http://dx.doi.org/10.1006/meth.2001.1262.


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Total RNA isolation, reverse transcription, and qPCR amplification in Thesis: Serum MicroRNAs in buffalo cows: potential biomarkers of pregnancy by Capurro M., 2019 is licensed under a Creative Commons Attribution 4.0 International License.
Permissions beyond the scope of this license may be available at http://indice.spvet.it/adv.html.