Biblioteca Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche
Sanità Pubblica Veterinaria: Numero 112, Febbraio 2019 [http://www.spvet.it/] ISSN 1592-1581
Smart Poster: SERUM MICRORNAs IN BUFFALO COWS: POTENTIAL BIOMARKERS OF PREGNANCY - Micro RNA serici in femmine di bufalo: potenziali biomarker di gravidanza. [Ver. 1.0]
Tesista: Michela Capurro; Relatore: Danilo Zampini; Correlatore: Gabriella Guelfi, aoul Ciappelloni
MicroRNA detection and differential expression analysis
To normalize qPCR data four candidate reference miRNAs were tested.
Updated bibliography
Andersen, C.L., Jensen, J.L., Ørntoft, T.F., 2004. Normalization of real-time quantitative
reverse transcription-PCR data: a model-based variance estimation approach to
identify genes suited for normalization, applied to bladder and colon cancer data sets.
Cancer Res. 64, 5245–5250. http://dx.doi.org/10.1158/0008-5472.CAN-04-0496.
Pfaffl, M.W., Tichopad, A., Prgomet, C., Neuvians, T.P., 2004. Determination of stable
housekeeping genes, differentially regulated target genes and sample integrity:
BestKeeper–excel-based tool using pair-wise correlations. Biotechnol. Lett. 26,
509–515.
Silver, N., Best, S., Jiang, J., Thein, S.L., 2006. Selection of housekeeping genes for gene
expression studies in human reticulocytes using real-time PCR. BMC Mol. Biol. 7, 33.
http://dx.doi.org/10.1186/1471-2199-7-33.
Wan, P., Tang, Y., Yuan, S., He, J., Wang, W., Lai, F., Fu, Q., 2017. Reference genes for
quantitative real-time PCR analysis in symbiont Entomomyces delphacidicola of
Nilaparvata lugens (Stal). Sci. Rep. 7http://dx.doi.org/10.1038/srep42206. (Article
number: 42206).
Xie, F., Xiao, P., Chen, D., Xu, L., Zhang, B., 2012. miRDeepFinder: a miRNA analysis tool
for deep sequencing of plant small RNAs. Plant Mol. Biol. http://dx.doi.org/10.1007/
s11103-012-9885-2.
Vandesompele, J., De Preter, K., Pattyn, F., Poppe, B., Van Roy, N., De Paepe, A.,
Speleman, F., 2002. Accurate normalization of real-time quantitative RT-PCR data by
geometric averaging of multiple internal control genes. Genome Biol. 3
RESEARCH0034.