Biblioteca Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche
Sanità Pubblica Veterinaria: Numero 112, Febbraio 2019 [http://www.spvet.it/] ISSN 1592-1581

torna all'indice della tesi

Smart Poster: SERUM MICRORNAs IN BUFFALO COWS: POTENTIAL BIOMARKERS OF PREGNANCY - Micro RNA serici in femmine di bufalo: potenziali biomarker di gravidanza. [Ver. 1.0]
Tesista: Michela Capurro; Relatore: Danilo Zampini; Correlatore: Gabriella Guelfi, aoul Ciappelloni





MicroRNA detection and differential expression analysis

To normalize qPCR data four candidate reference miRNAs were tested.


Updated bibliography

Andersen, C.L., Jensen, J.L., Ørntoft, T.F., 2004. Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 64, 5245–5250. http://dx.doi.org/10.1158/0008-5472.CAN-04-0496.

Pfaffl, M.W., Tichopad, A., Prgomet, C., Neuvians, T.P., 2004. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper–excel-based tool using pair-wise correlations. Biotechnol. Lett. 26, 509–515.

Silver, N., Best, S., Jiang, J., Thein, S.L., 2006. Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. BMC Mol. Biol. 7, 33. http://dx.doi.org/10.1186/1471-2199-7-33.

Wan, P., Tang, Y., Yuan, S., He, J., Wang, W., Lai, F., Fu, Q., 2017. Reference genes for quantitative real-time PCR analysis in symbiont Entomomyces delphacidicola of Nilaparvata lugens (Stal). Sci. Rep. 7http://dx.doi.org/10.1038/srep42206. (Article number: 42206).

Xie, F., Xiao, P., Chen, D., Xu, L., Zhang, B., 2012. miRDeepFinder: a miRNA analysis tool for deep sequencing of plant small RNAs. Plant Mol. Biol. http://dx.doi.org/10.1007/ s11103-012-9885-2.

Vandesompele, J., De Preter, K., Pattyn, F., Poppe, B., Van Roy, N., De Paepe, A., Speleman, F., 2002. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 3 RESEARCH0034.



Creative Commons License
MicroRNA detection and differential expression analysis in thesis: Serum MicroRNAs in buffalo cows: potential biomarkers of pregnancy by Capurro M., 2019 is licensed under a Creative Commons Attribution 4.0 International License.
Permissions beyond the scope of this license may be available at http://indice.spvet.it/adv.html.